tidesurf documentation#
This is the documentation for tidesurf, a Tool for IDentification and Enumeration of Spliced and Unspliced Read Fragments, implemented in Python. It enables the quantification of spliced and unspliced reads from 10x Genomics 5’ and 3’ scRNA-seq data, using the 10x Genomics Cell Ranger output.
Note
This project and its documentation are under active development.
Note
In the future, we aim to support other single-cell RNA-seq data formats, such as Smart-seq2.
Contents:
Citation#
If you use tidesurf in your research, please cite the following publication:
Schleicher, J.T., and Claassen, M. (2025). Accurate quantification of spliced and unspliced transcripts for single-cell RNA sequencing with tidesurf. bioRxiv 2025.01.28.635274; DOI: 10.1101/2025.01.28.635274.